Determination of substituent effects on the proton affinities of natural nucleosides by the kinetic method. (15th January 2021)

• Record Type: Journal Article
• Authors: Liguori, Angelo; Napoli, Anna; Sindona, Giovanni
• Other Names: Giorgi Gianluca guestEditor.; Napoli Anna guestEditor.
• Is Part Of: Journal of mass spectrometry. Volume 56:Number 1(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2021-01-15
• Subjects: Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.4677
• Languages: English
• ISSNs: 1076-5174

Abstract

Abstract: The kinetics of the unimolecular dissociations of proton‐bound dimers produced by fast‐atom bombardment from nucleosides and reference amines enables the evaluation of the proton affinities (PAs) of ribonucleosides. The PAs of cytosine, guanosine, adenosine, uridine, and deoxyuridine have been thus determined. These values and those already available for the corresponding DNA homologues allow the evaluation of the effect of the hydroxyl group in position 2′ of the sugar moiety, which lowers the PAs of RNA nucleosides by 0.6–1 kcal/mol, and of the methyl group in position 5 of the thymine ring, which enhances the basicity of deoxythymidine over deoxyuridine by 0.6 kcal/mol.