Stem cell‐derived enteroid cultures as a tool for dissecting host‐parasite interactions in the small intestinal epithelium. (5th August 2020)

• Record Type: Journal Article
• Authors: Hares, Miriam F.; Tiffney, Ellen‐Alana; Johnston, Luke J.; Luu, Lisa; Stewart, Christopher J.; Flynn, Robin J.; Coombes, Janine L.
• Other Names: Helmby Helena guestEditor.; Hafalla Julius Clemence guestEditor.
• Is Part Of: Parasite immunology. Volume 43:Number 2(2021)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-08-05
• Subjects: apicomplexan -- Cryptosporidium parvum -- enteroid -- intestinal epithelium -- Neospora caninum -- organoid -- Toxoplasma gondii; Veterinary parasitology -- Immunological aspects -- Periodicals; Host-parasite relationships -- Immunological aspects -- Periodicals; 571.96
• DOI: 10.1111/pim.12765
• Languages: English
• ISSNs: 0141-9838

Abstract

Abstract: Toxoplasma gondii and Cryptosporidium spp. can cause devastating pathological effects in humans and livestock, and in particular to young or immunocompromised individuals. The current treatment plans for these enteric parasites are limited due to long drug courses, severe side effects or simply a lack of efficacy. The study of the early interactions between the parasites and the site of infection in the small intestinal epithelium has been thwarted by the lack of accessible, physiologically relevant and species‐specific models. Increasingly, 3D stem cell‐derived enteroid models are being refined and developed into sophisticated models of infectious disease. In this review, we shall illustrate the use of enteroids to spearhead research into enteric parasitic infections, bridging the gap between cell line cultures and in vivo experiments.