Enhancement in the Capture Efficiency of Magnetoelastic Biosensors for Salmonella Using a Dilution Method. (25th October 2017)

• Record Type: Journal Article
• Authors: Xi, Jianguo; Horikawa, Shin; Chen, I-Hsuan; Du, Songtao; Liu, Yuzhe; Lu, Xu; Suh, Sang-Jin; Huang, Tung-Shi; Chin, Bryan A
• Is Part Of: ECS transactions. Volume 80:Number 10(2017)
• Page Start: 1549
• Page End: 1555
• Publication Date: 2017-10-25
• Subjects: Electrochemistry -- Periodicals; Electrochemistry; Periodicals; Electronic journals; Electronic journal; 541.37
• DOI: 10.1149/08010.1549ecst
• Languages: English
• ISSNs: 1938-5862

Abstract

Abstract : This paper investigates three different washing methods to improve the capture efficiency of E2 phage-coated magnetoelastic (ME) biosensors for Salmonella Typhimurium . ME biosensors with pre-determined resonant frequencies were exposed to Salmonella Typhimurium of 5x10 4 cfu/ml for 1 hr. E2 phage immobilized on the biosensors was responsible for specifically capturing the bacteria. After this capture step, the biosensors were washed using three different washing methods, pipette washing, magnetic bar washing, and dilution washing. The dilution method was found to result in the highest capture efficiency of the bacteria and greatest resonant frequency shift of the sensors, which means the lowest loss rate of Salmonella Typhimurium .