Fluorometric Bio-Sniffer (Gas Phase Biosensor) for Breath Acetone as a Volatile Product of Lipid Metabolism. (16th August 2016)

• Record Type: Journal Article
• Authors: Chien, Po-Jen; Ye, Ming; Suzuki, Takuma; Toma, Koji; Arakawa, Takahiro; Mitsubayashi, Kohji
• Is Part Of: ECS transactions. Volume 75:Number 16(2016)
• Page Start: 47
• Page End: 51
• Publication Date: 2016-08-16
• Subjects: Electrochemistry -- Periodicals; Electrochemistry; Periodicals; Electronic journals; Electronic journal; 541.37
• DOI: 10.1149/07516.0047ecst
• Languages: English
• ISSNs: 1938-5862

Abstract

Abstract : A highly sensitive acetone bio-sniffer (biochemical gas sensor) for assessment of lipid metabolism utilizing reverse reaction of secondary alcohol dehydrogenase was fabricated and demonstrated. This bio-sniffer consisted of a NADH fluorescence detection system, a flow-cell section, and an enzyme-immobilized membrane. This bio-sniffer presented rapid response, highly sensitivity and selectivity. The analyses of breath acetone concentrations during fasting and aerobic exercise were also demonstrated. The results showed that the breath acetone concentration would increase during long-time fasting and aerobic exercise. This acetone bio-sniffer provides a new kind of analytical tool for non-invasive evaluation of human lipid metabolism and it is also expected to use for the clinical applications such as monitoring the progression of diabetes.