Sensitivity Enhancement of Electrochemical Biosensor for Point of Care (POC) Applications: Vi Antigen Detection as a Case Study. Issue 1 (14th January 2021)

• Record Type: Journal Article
• Authors: Maurya, Ranjan Kumar; Alam, M. A.; Ahamad, Nadeem; Kishore, Kaushal; Prajesh, Rahul; Choudhary, Megha; Bhalla, Vijayender; Agarwal, Ajay
• Is Part Of: Journal of the Electrochemical Society. Volume 168:Issue 1(2021)
• Publication Date: 2021-01-14
• Subjects: Electrochemical sensor -- Vi antigen -- POC -- Microfabrication -- Biosensor -- mechanical vibration; Electrochemistry -- Periodicals; 541.3705
• DOI: 10.1149/1945-7111/abd929
• Languages: English
• ISSNs: 0013-4651

Abstract

Abstract : A novel approach for signal enhancement of electrochemical biosensors by incorporating mechanical vibrations has been developed. We report the electrochemical study of the ferricyanide and ferrocyanide Fe CN 6 − 3 / − 4 a redox couple, at room temperature (∼25 °C), under the effect of mechanical vibrations of different frequencies applied to the sensor, for sensitivity enhancement. The experimental results showed a sensitivity enhancement of ∼332% (from 3.125 nA μ M − 1 to 13.5 nA μ M − 1 ) at 88.75 Hz of vibration. This novel approach of signal sensitivity enhancement is also validated with antibody immobilization-based Vi antigen detection for typhoid assessment. The sensitivity enhancement up to ∼15% is achieved for Vi antigen detection under the mechanical vibration of 120 Hz. The sensor is fabricated using microfabrication technology. The vibration-assisted ultrasensitive biosensing platform is also prototyped as a portable and Internet of Thing (IoT) enabled device, suitable for point-of-care applications. Detailed features of the prototype along with the test results are elaborated in the paper.