Interaction of silver nanoparticles with plasma transport proteins: A systematic study on impacts of particle size, shape and surface functionalization. (1st February 2021)

• Record Type: Journal Article
• Authors: Barbir, Rinea; Capjak, Ivona; Crnković, Tea; Debeljak, Željko; Domazet Jurašin, Darija; Ćurlin, Marija; Šinko, Goran; Weitner, Tin; Vinković Vrček, Ivana
• Is Part Of: Chemico-biological interactions. Volume 335(2021)
• Publication Date: 2021-02-01
• Subjects: Nano-bio interface -- Albumin -- α-1-Acid glycoprotein -- Binding constants -- Conformational change -- Protein corona; Biochemistry -- Periodicals; Toxicological chemistry -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biochimie -- Périodiques; Toxicologie biochimique -- Périodiques; 572
• DOI: 10.1016/j.cbi.2020.109364
• Languages: English
• ISSNs: 0009-2797

Abstract

Abstract: Metallic nanoparticles are an important and widely used materials in development of nano-enabled medicine. For that reason, their interaction with biological molecules has to be systematically examined, as use of nanoparticles can lead to altered biological functions. In this study, we evaluated the interaction between silver nanoparticles (AgNPs) and two important plasma transport proteins - albumin and α-1-acid glycoprotein. To investigate comprehensively how different physico-chemical properties impact interaction of proteins with nanosurface, AgNPs of different size, shape and surface coating was prepared. The study was conducted using UV–Vis absorption, fluorescence, inductively coupled plasma mass spectrometry, circular dichroism spectroscopy, transmission electron microscopy, dynamic and electrophoretic light scattering techniques. The results showed significant complexities of the nano-bio interface and binding affinities of proteins onto surface of different AgNPs, which were affected by both AgNPs and protein properties. The most significant role on AgNPs-protein interaction had the coating agents used for AgNPs surface stabilization. Our findings should improve safe-by-design approach to development of the metallic nanomaterials for medical use. Graphical abstract: Image 1 Highlights: Interaction of AgNPs with albumin and α-1-acid glycoprotein is presented Nano-bio interface is affected by size, shape and surface coating of different AgNPs Complexity of events are also related to structure, properties and functionality of proteins Coating agents used for AgNPs stabilization has major role at this nano-bio interface