Identification of aldehyde oxidase 3 as a binding protein for squid ink polysaccharides using magnetic nanoparticles. Issue 6 (18th January 2021)

• Record Type: Journal Article
• Authors: Lin, Zhen; Tan, Xiaohui; Li, Fangping; Zhang, Yu; Luo, Ping; Lin, Xuan; Liu, Huazhong
• Is Part Of: RSC advances. Volume 11:Issue 6(2021)
• Page Start: 3596
• Page End: 3602
• Publication Date: 2021-01-18
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/d0ra09222c
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : Superparamagnetic nanometer beads bonding SIP was employed to pull down the binding protein from the liver of mouse, which was identified as aldehyde oxidase 3. By means of enzyme kinetics analysis, SIP was found to activate AOX3 enzyme activity. Abstract : To explore the interactive molecules of squid ink polysaccharides (SIP) for further understanding the action mechanisms of SIP bio-function, this study prepared SIP binding proteins from mouse liver using superparamagnetic nanometer beads. Michaelis–Menten constant ( K m ) was detected from a Lineweaver–Burk double reciprocal plot to assess effect of SIP on activity of aldehyde oxidase (AOX). Results showed that three proteins, AOX-3, regucalcin (RGN) and α1-antitrypsin (A1AT3) were separated from mouse liver by magnetic nanoparticles conjugated with SIP. Contents of AOX-3 were much more than RGN and A1AT3. SIP (0.5 mg mL −1 ) reduced K m value of aldehyde oxidase of mouse liver from 91.79 μmol L −1 to 43.70 μmol L −1 .