Appropriate regulation of magnesium on hydroxyapatite crystallization in simulated body fluids. Issue 3 (15th December 2020)

• Record Type: Journal Article
• Authors: Jiang, Shuqin; Cao, Yuanyuan; Zong, Chenxi; Pang, Yuanfeng; Sun, Zhiwen
• Is Part Of: CrystEngComm. Volume 23:Issue 3(2021)
• Page Start: 678
• Page End: 683
• Publication Date: 2020-12-15
• Subjects: Crystals -- Periodicals; Crystal growth -- Periodicals; Crystallography -- Periodicals; Cristaux -- Périodiques; Cristaux -- Croissance -- Périodiques; Cristallographie -- Périodiques; 548
• DOI: 10.1039/d0ce01421d
• Languages: English
• ISSNs: 1466-8033

Abstract

Abstract : The regulation effect of Mg 2+ on HAP crystallization is closely related to the adding time of Mg 2+ . The introduction of Mg 2+ in the ACP unstable stage is unable to inhibit HAP crystallization. Abstract : Amorphous calcium phosphate (ACP), as a precursor phase of hydroxyapatite (HAP, the main inorganic mineral of bone) crystallization in a biological system is well documented. Numerous studies have shown that Mg 2+ can chemically inhibit the transformation of ACP to HAP. Importantly, the inhibition effect is closely related to the addition time of Mg 2+ for bone mineralization in vivo . However, Mg 2+ is also widely used to promote bone growth and regeneration in the biomedical field. So, the regulatory role of Mg 2+ in HAP crystallization remains elusive. In this study, the detailed effect of Mg 2+ (especially the adding time) on ACP-mediated HAP crystallization is revealed in simulated body fluids. We reported that adding Mg 2+ before ACP formation or in ACP stable stage can effectively inhibit HAP crystallization; however, in the near phase transformation stage (ACP is unstable), Mg 2+ does not affect HAP crystallization. In particular, surface associated Mg 2+ on ACP (compared to Mg 2+ incorporation) is more suppressive to HAP crystallization. This results indicates that appropriate regulation of Mg 2+ adding time is vital for amorphous mediated biomineralization, which sheds new light on HAP crystallization regulation.