Development and application of a real-time RT-PCR assay to rapidly detect H2 subtype avian influenza A viruses. (May 2021)

• Record Type: Journal Article
• Authors: Xiao, Yixin; Yang, Fan; Liu, Fumin; Yao, Hangping; Wu, Nanping; Wu, Haibo
• Is Part Of: Journal of veterinary diagnostic investigation. Volume 33:Number 3(2021)
• Page Start: 577
• Page End: 581
• Publication Date: 2021-05
• Subjects: avian influenza A virus -- H2 subtype -- real-time RT-PCR; Veterinary medicine -- Diagnosis -- Periodicals; 636.0896075
• DOI: 10.1177/1040638721994810
• Languages: English
• ISSNs: 1040-6387

Abstract

The H2 subtypes of avian influenza A viruses (avian IAVs) have been circulating in poultry, and they have the potential to infect humans. Therefore, establishing a method to quickly detect this subtype is pivotal. We developed a TaqMan minor groove binder real-time RT-PCR assay that involved probes and primers based on conserved sequences of the matrix and hemagglutinin genes. The detection limit of this assay was as low as one 50% egg infectious dose (EID50 )/mL per reaction. This assay is specific, sensitive, and rapid for detecting avian IAV H2 subtypes.