Photo-tethered molecular beacons for superior light-induction. Issue 5 (21st December 2020)

• Record Type: Journal Article
• Authors: Klimek, Robin; Wang, Mantian; McKenney, Vivien R.; Schuman, Erin M.; Heckel, Alexander
• Is Part Of: Chemical communications. Volume 57:Issue 5(2021)
• Page Start: 615
• Page End: 618
• Publication Date: 2020-12-21
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/d0cc06704k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Photolabile circularization of molecular beacons via backbone phosphates leads to superior probes to study spatiotemporal aspects of RNA in cells. Abstract : We developed a superior class of light-activatable molecular beacons with photo-tethered loop regions. Two simple modifications and probe cyclisation prevent the molecular beacon from hybridising with the target RNA before light-activation. Full activity of the molecular beacon is elicited upon illumination with 365 nm light.