A biomimetic model of 3D fluid extracellular macromolecular crowding microenvironment fine-tunes ovarian cancer cells dissemination phenotype. (February 2021)

• Record Type: Journal Article
• Authors: Bascetin, Rümeyza; Laurent-Issartel, Carine; Blanc-Fournier, Cécile; Vendrely, Charlotte; Kellouche, Sabrina; Carreiras, Franck; Gallet, Olivier; Leroy-Dudal, Johanne
• Is Part Of: Biomaterials. Volume 269(2021)
• Publication Date: 2021-02
• Subjects: Macromolecular crowding -- Extracellular matrix -- Ovarian cancer -- Spheroid -- Dissemination -- Migration; Dx dextran -- ECM extra cellular matrix -- FBS fetal bovine serum -- Fc ficoll -- FVO fractional volume occupancy -- mAb monoclonal antibody -- MMC macromolecular crowding -- NSI nuclear shape index -- OCC ovarian cancer cell -- pAb polyclonal antibody -- SDS sodium dodecyl sulfate -- TBS Tris buffer saline -- TRIS 2-amino-2-hydroxyméthylpropane-1, 3-diol; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2020.120610
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: An early fundamental step in ovarian cancer progression is the dissemination of cancer cells through liquid environments, one of them being cancer ascites accumulated in the peritoneal cavity. These biological fluids are highly crowded with a high total macromolecule concentration. This biophysical property of fluids is widely used in tissue engineering for a few decades now, yet is largely underrated in cancer biomimetic models. To unravel the role of fluids extracellular macromolecular crowding (MMC), we exposed ovarian cancer cells (OCC) to high molecular weight inert polymer solutions. High macromolecular composition of extracellular liquid presented a differential effect: i ) it impeded non-adherent OCC aggregation in suspension and, decreased their adhesion; ii ) it promoted adherent OCC migration by decreasing extracellular matrix deposition. Besides, there seemed to be a direct link between the extracellular MMC and intracellular processes, especially the actin cytoskeleton organization and the nucleus morphology. In conclusion, extracellular fluid MMC orients OCC dissemination phenotype. Integrating MMC seems crucial to produce more relevant mimetic 3D in vitro fluid models to study ovarian dissemination but also to screen drugs. Graphical abstract: Image 1 Highlights: Ovarian cancer cells display differential behaviors in crowded extracellular fluid. Extracellular macromolecular crowding impedes ovarian cancer cells aggregation. Extracellular macromolecular crowding affects migration of ovarian cancer cells. Extracellular macromolecular crowding fine-tunes intracellular actin and nucleus. Extracellular macromolecular crowding is a driving force for relevant cancer model.