A dual-modal PET/near infrared fluorescent nanotag for long-term immune cell tracking. (February 2021)

• Record Type: Journal Article
• Authors: Harmsen, Stefan; Medine, Emin Ilker; Moroz, Maxim; Nurili, Fuad; Lobo, Jose; Dong, Yiyu; Turkekul, Mezruh; Pillarsetty, Naga Vara Kishore; Ting, Richard; Ponomarev, Vladimir; Akin, Oguz; Aras, Omer
• Is Part Of: Biomaterials. Volume 269(2021)
• Publication Date: 2021-02
• Subjects: Dual-modal -- PET -- Near-infrared fluorescence -- Immune cell labeling -- Nanomedicine -- Tracking; BLI bioluminescent imaging -- CAR chimeric antigen receptor -- iTLC instant thin layer radio-chromatography -- NIRF near infrared fluorescent -- PET positron emission tomography -- RO retro-orbital -- SPION superparamagnetic iron oxide nanoparticle; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2020.120630
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Adoptive cell transfer of targeted chimeric antigen receptor (CAR) T cells has emerged as a highly promising cancer therapy. The pharmacodynamic action or CAR T cells is closely related to their pharmacokinetic profile; because of this as well as the risk of non-specific action, it is important to monitor their biodistribution and fate following infusion. To this end, we developed a dual-modal PET/near infrared fluorescent (NIRF) nanoparticle-based imaging agent for non-genomic labeling of human CAR T cells. Since the PET/NIRF nanoparticles did not affect cell viability or cytotoxic functionality and enabled long-term whole-body CAR T cell tracking using PET and NIRF in an ovarian peritoneal carcinomatosis model, this platform is a viable imaging technology to be applied in other cancer models.