Clinical hematological and biochemical parameters in Swiss, BALB/c, C57BL/6 and B6D2F1 Mus musculus. Issue 4 (18th December 2020)

• Record Type: Journal Article
• Authors: Silva‐Santana, Giorgio; Bax, Juliet Cunha; Fernandes, Débora Cristina Silva; Bacellar, Daniela Tendler Leibel; Hooper, Cleber; Dias, Alexandre Alves Souza Oliveira; Silva, Cristina Barbosa; de Souza, Aline Moreira; Ramos, Simone; Santos, Ricardo Alexandre; Pinto, Thainara Ramos; Ramão, Mariana Antunes; Mattos‐Guaraldi, Ana Luíza
• Is Part Of: Animal models and experimental medicine. Volume 3:Issue 4(2020)
• Page Start: 304
• Page End: 315
• Publication Date: 2020-12-18
• Subjects: B6D2F1 -- BALB/c -- biochemical -- C57BL/6 -- hematological -- Swiss; Laboratory animals -- Periodicals; Diseases -- Animal models -- Periodicals; Animal models in research -- Periodicals; Veterinary medicine -- Periodicals; Laboratory Animal Science; Disease Models, Animal; Animals, Laboratory; Animal Welfare; Veterinary Medicine; Animal models in research; Diseases -- Animal models; Laboratory animals; Veterinary medicine; Periodicals; Fulltext; Internet Resources; Periodicals; Periodicals; 616.0273
• DOI: 10.1002/ame2.12139
• Languages: English
• ISSNs: 2576-2095

Abstract

Abstract: Background: Animal models are widely used in scientific research in order to obtain information from a whole organism under a specific set of experimental conditions. Various lineages of mice have been used to investigate diseases and new therapeutic strategies, and, consequently, hematological and biochemical tests in these laboratory animals are essential to validate scientific studies. Our study seeks to establish reference values for hematological and biochemical parameters of four lineages of mice. Methods: We evaluated the hematological and biochemical profiles of 20 males and 20 females from the lineages Swiss (heterogeneous), BALB/c and C57BL/6 (isogenic), and B6D2F1 (hybrid), totaling 160 mice. Analysis were standardized using the systems pocH‐100iV Diff ™ for 19 hematological parameters and VITROS ® 350 for 12 biochemical parameters. Results: Results are shown as means and standard deviation, grouped by lineage and genre. Comparing the values obtained in this study with the values from previous studies, some variations were detected, which could be explained by differences in methodologies or individual variability. Conclusion: Thus our study shows that knowledge and disclosure of the values of physiological parameters of laboratory animals is necessary, and emphasises the importance of considering variations influenced by gender, lineage and genotype in the choice of the best experimental model.