Effects of paralytic shellfish poisoning toxin-producing dinoflagellate Gymnodinium catenatum on the marine copepod Tigriopus japonicus. (February 2021)

• Record Type: Journal Article
• Authors: Han, Jeonghoon; Park, Joon Sang; Park, Yeun; Lee, Jihoon; Shin, Hyun Ho; Lee, Kyun-Woo
• Is Part Of: Marine pollution bulletin. Volume 163(2021)
• Publication Date: 2021-02
• Subjects: Harmful algae bloom -- Swimming behavior parameters -- Detoxification -- Cytochrome P450s -- Glutathione-S transferases; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2020.111937
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstracts: To understand how the marine copepod Tigriopus japonicus responds to the toxic marine dinoflagellate Gymnodinium catenatum, we assessed acute toxicity and investigated swimming behavior parameters (e.g., swimming speed, swimming path trajectory, and swimming distance) in response to G. catenatum exposure. In addition, the mRNA expression levels of detoxification-related genes (e.g., phase I cytochrome P450 [ CYP ] and phase II glutathione- S transferase [ GST ]) were measured in G. catenatum -exposed copepods. No significant change in survival was observed in response to G. catenatum, but swimming speed was significantly decreased (P < 0.05) at a high concentration of G. catenatum (600 cells/mL). Furthermore, the swimming distance was significantly decreased (P < 0.05) compared to that of the control at 600 cells/mL G. catenatum, while no significant change in swimming path trajectory was observed, suggesting that G. catenatum potentially has adverse effects on the swimming behavior of T. japonicus . In addition, the transcriptional regulation of T. japonicus CYP s and - GST s were significantly upregulated and downregulated (P < 0.05), respectively, in response to G. catenatum . In particular, certain genes (e.g., CYP s [ CYP307E1, CYP3041A1, and CYP3024A2 ] and GST s [ GST - kappa, GST - mu5, and GST - omega ]) were significantly induced (P < 0.05) by G. catenatum, suggesting that these genes likely play a critical role in detoxification mechanisms and might be useful as potential molecular biomarkers in response to G. catenatum exposure. Overall, these results elucidate the potential impacts of the dinoflagellate G. catenatum on the swimming behavior and detoxification system of the marine copepod T. japonicus . Highlights: No significant change in survival was observed in response to G. catenatum . Swimming speed and swimming distance were significantly decreased at 600 cells/mL G. catenatum . No significant change in swimming path trajectory was observed response to G. catenatum . CYP s and GST s were down- or upregulated in response to G. catenatum .