Comparative proteomic analysis reveals insights into the dynamic responses of maize (Zea mays L.) to Setosphaeria turcica infection. (March 2021)

• Record Type: Journal Article
• Authors: Liu, Yuwei; Gong, Xiaodong; Zhou, Qihui; Liu, Yajie; Liu, Zhenpan; Han, Jianmin; Dong, Jingao; Gu, Shouqin
• Is Part Of: Plant science. Volume 304(2021)
• Publication Date: 2021-03
• Subjects: NCLB Northern corn leaf blight -- PTI pattern triggered immunity -- ETI effector-triggered immunity -- PRR pattern recognition receptor -- NBS-LRR nucleotide-binding site-leucine-rich repeat -- MS/MS tandem mass spectrometry -- TMT tandem mass tags -- HR hypersensitivity reaction -- DEP differentially expressed protein -- GST glutathione S-transferase -- JA jasmonic acid -- SA salicylic acid -- PRM parallel reaction monitoring -- BX benzoxazinoid -- DIMBOA-Glc 2, 4-dihydroxy-7-methoxy-2H-1, 4-benzoxazin-3(4H)-oneGlc -- HDMBOA-Glc 2-hydroxy-4, 7-dimethoxy-1, 4-benzoxazin-3-one-Glc -- DIM2BOA-Glc 2, 4-dihydroxy-7, 8-dimethoxy-1, 4-benzoxazin-3-one; Maize -- Setosphaeriaturcica -- NCLB -- Proteome; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2020.110811
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: First reported proteomic study of maize and Setosphaeria turcica interaction. Insight into the dynamic reprogramming of leaves proteomes during S. turcica infection. Total 1331 DEPs were identified and 14 of which were verified by parallel reaction monitoring experiment. The biosynthesis of phenylpropanoid and BX and JA signal pathway were involved in the response of maize to S. turcica infection. GST family proteins could contribute to the resistance of maize against S. turcica . Abstract: Maize ( Zea mays L.) production is severely affected by northern corn leaf blight (NCLB), which is a destructive foliar disease caused by Setosphaeria turcica . In recent years, studies on the interaction between maize and S. turcica have been focused at the transcription level, with no research yet at the protein level. Here, we applied tandem mass tag labelling and liquid chromatography–tandem mass spectrometry to investigate the proteomes of maize leaves at 24 h and 72 h post-inoculation (hpi) with S. turcica. In total, 4740 proteins encoded by 4711 genes were quantified in this study. Clustering analyses provided an understanding of the dynamic reprogramming of leaves proteomes by revealing the functions of different proteins during S. turcica infection. Screening and classification of differentially expressed proteins (DEPs) revealed that numerous defense-related proteins, including defense marker proteins and proteins related to the phenylpropanoid lignin biosynthesis, benzoxazine biosynthesis and the jasmonic acid signalling pathway, participated in the defense responses of maize to S. turcica infection. Furthermore, the earlier induction of GST family proteins contributed to the resistance to S. turcica . In addition, the protein–protein interaction network of DEPs suggests that some defense-related proteins, for example, ZmGEB1, a hub node, play key roles in defense responses against S. turcica infection. Our study findings provide insight into the complex responses triggered by S. turcica at the protein level and lay the foundation for studying the interaction process between maize and S. turcica infection.