Chitosan and gelatin biopolymer supplemented with mesenchymal stem cells (Velgraft®) enhanced wound healing in goats (Capra hircus): Involvement of VEGF, TGF and CD31. Issue 1 (February 2021)

• Record Type: Journal Article
• Authors: Shukla, Amit; Choudhury, Soumen; Chaudhary, Gaurav; Singh, Vandana; Prabhu, Shyama N.; Pandey, Siddharth; Garg, Satish Kumar
• Is Part Of: Journal of tissue viability. Volume 30:Issue 1(2021)
• Page Start: 59
• Page End: 66
• Publication Date: 2021-02
• Subjects: Mesenchymal stem cells -- Wound healing -- Cytokines -- Tissue regeneration; Wounds and injuries -- Periodicals; Ulcers -- Periodicals; Bedsores -- Periodicals; Bedsores; Ulcers; Wounds and injuries; Electronic journals; Periodicals; 617.1406
• DOI: 10.1016/j.jtv.2020.12.002
• Languages: English
• ISSNs: 0965-206X

Abstract

Abstract: Aim: Cell-based therapy has emerged as promising strategy for chronic and impaired wounds treatment. Current research is focused on developing biomaterial systems that act as a niche for mesenchymal stem cells (MSCs) to promote wound healing through paracrine molecular cascading. This study was aimed to evaluate the wound healing potential of Velgraft, a ready-to-use biodegradable artificial skin substitute, on excision wound in goats. Materials and methods: Twelve male goats were randomized divided in to three groups of four animals each. After infliction of surgical wound, Velgraft and Soframycin were applied on wounds of the animals of Groups II and III while Group I (sham operated) served as control. Wound diameters were measured at pre-defined time-points for determination of progressive wound healing up to 28 days. Skin sections were stained using Hematoxylin and eosin (H&E) for examining the histoarchitectural changes, Masson trichome staining for ascertaining collagen synthesis and immunohistochemistry for expression of CD31, VEGF and TGF-β1 proteins to determine post-treatment angiogenesis in the inflicted wounds. Results: Velgraft application appreciably enhanced wound closure by day 21 which was confirmed through restoration of the normal skin architecture as evident based on histopathological examination and characterized by complete regeneration of epidermal layers, collagen fibers, blood capillaries and hair follicular formation. Stimulation of angiogenesis markers was also observed at different time-points post-Velgraft application; which is suggestive of the improved angiogenesis and vasculogenesis. Conclusion: Velgraft facilitates wound healing by augmenting early wound closure, enhancing collagen synthesis and deposition, trichosis development and promoting revascularization and epidermal layers restoration. Highlights: Velgraft promotes wound healing by enhancing vascularization and re-epithelization. Velgraft showed hair follicle formation and epidermal regeneration by H&E staining. Masson trichome staining depicted higher collagen synthesis on Velgraft application. Stimulation of CD31, VEGF, TGF-β1expression was observed post Velgraft application.