Octominin: An antibacterial and anti-biofilm peptide for controlling the multidrug resistance and pathogenic Streptococcus parauberis. Issue 110 (March 2021)

• Record Type: Journal Article
• Authors: Thulshan Jayathilaka, E.H.T.; Liyanage, T.D.; Rajapaksha, D.C.; Dananjaya, S.H.S.; Nikapitiya, Chamilani; Whang, Ilson; De Zoysa, Mahanama
• Is Part Of: Fish & shellfish immunology. Issue 110(2021)
• Page Start: 23
• Page End: 34
• Publication Date: 2021-03
• Subjects: Antibacterial -- Antimicrobial peptides -- Anti-Biofilm -- Octominin -- Octopus minor -- Streptococcus parauberis; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.12.017
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Streptococcus parauberis is a pathogenic gram-positive bacterium that causes streptococcosis infection in fish. Since S. parauberis is becoming resistant to multiple antibiotics, the development of alternatives, such as antimicrobial peptides, has gained great attention. Octominin, derived from the defense protein of Octopus minor, showed a significant antimicrobial activity against multidrug resistance S. parauberis, with a minimum inhibitory concentration (MIC) and a minimum bactericidal concentration (MBC) of 50 and 100 μg/mL, respectively. Furthermore, time-kill kinetics, agar diffusion, and bacterial viability assays confirmed the concentration-dependent antibacterial activity of Octominin against S. parauberis . Field emission scanning electron microscopy analysis showed morphological and ultra-structural changes in S. parauberis upon Octominin treatment. Moreover, Octominin treatment demonstrated changes in membrane permeability, induced reactive oxygen species (ROS), and its binding ability to genomic DNA, suggesting its strong bactericidal activity with multiple modes of action. We confirmed the inhibition of biofilm formation and the eradication of existing biofilms in a concentration-dependent manner. Additionally, Octominin on S . parauberis at transcriptional level exhibited downregulation of membrane formation ( pgsA and cds1 ), DNA repairing ( recF ), biofilm formation ( pgaB and epsF ) genes, while upregulation of ROS detoxification ( sodA ) and DNA protecting ( ahpF ) related genes. An in vivo study confirmed a significantly ( P < 0.05) higher relative percentage survival in Octominin-treated larval zebrafish exposed to S. parauberis (93.3%) compared to the control group (20.0%). Collectively, our results confirm that Octominin could be a potential antibacterial and anti-biofilm agent against S. parauberis. Graphical abstract: Image 1 Highlights: Octominin displayed strong antibacterial activity against Streptococcus parauberis. Octominin involved inhibition and eradication of biofilm in S. parauberis. S. parauberis exposed larval zebrafish are protected by Octominin treatment.