Evaluation of mesophilic Burkholderia sacchari, thermophilic Schlegelella thermodepolymerans and halophilic Halomonas halophila for polyhydroxyalkanoates production on model media mimicking lignocellulose hydrolysates. (April 2021)

• Record Type: Journal Article
• Authors: Kourilova, Xenie; Novackova, Ivana; Koller, Martin; Obruca, Stanislav
• Is Part Of: Bioresource technology. Volume 325(2021)
• Publication Date: 2021-04
• Subjects: Burkholderia sacchari -- Extremophiles -- Halomonas halophila -- Lignocellulose materials -- Microbial inhibitors -- Polyhydroxyalkanoates -- Schlegelella thermodepolymerans; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.124704
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Tested extremophiles overperformed B. sacchari in PHA production from lignocelluloses. H. halophila reached high PHA titers on hexoses-rich hydrolysates. S. thermodepolymerans preferred hydrolysates rich in pentoses. Both tested extremophiles were sensitive to microbial inhibitors. The utilization of extremophiles bring numerous advantages to the biotech process. Abstract: In this work, the mesophilic bacterium Burkholderia sacchari, the halophilic bacterium Halomonas halophila, and the thermophilic bacterium Schlegelella thermodepolymerans were evaluated with regards to their suitability for polyhydroxyalkanoates (PHA) production from model media mimicking lignocellulose hydrolysates. B. sacchari was capable of utilizing all the tested "model hydrolysates", yielding comparable PHA titers and turning out as very robust against lignocellulose-derived microbial inhibitors. On the contrary, H. halophila reached substantially higher PHA titers on hexoses-rich media, while S. thermodepolymerans preferred media rich in pentoses. Both extremophiles were more sensitive to microbial inhibitors than B. sacchari . Nevertheless, considering substantially higher PHA productivity of both extremophiles even in the presence of microbial inhibitors and also other positive factors associated with utilization of extremophiles, such as the reduced risk of microbial contamination, both H. halophila and S. thermodepolymerans are auspicious candidates for sustainable PHA production from abundantly available, inexpensive lignocelluloses.