The adverse effects of acrylamide exposure on the early development of marine medaka (Oryzias melastigma) and its mechanisms. (February 2021)

• Record Type: Journal Article
• Authors: Yue, Zonghao; Tian, Erli; Chen, Yanjuan; Luo, Liumin; Yang, Licheng; He, Le; Li, Lili; Wang, Jun
• Is Part Of: Marine pollution bulletin. Volume 163(2021)
• Publication Date: 2021-02
• Subjects: AA Acrylamide -- YE yolk sac edema -- PE pericardial edema -- SC spinal curvature -- Hb hemoglobin -- Mb myoglobin -- ANGPT1 angiopoietin 1 -- MMPs matrix metallopeptidases -- Egr early growth response gene -- Syt2 synaptotagmin-2 -- DEGs differentially expressed genes -- GO gene ontology -- KEGG kyoto encyclopedia of genes and genomes -- RNA-seq RNA sequencing; Acrylamide -- Marine medaka -- Early development -- RNA-sequencing; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2020.111875
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: Acrylamide (AA) can have deleterious effects on freshwater fish. However, its adverse effects on euryhaline fish are still unknown. In this study, embryos of Oryzias melastigma were exposed to different concentrations of AA to investigate its effect on early developmental disorders. After 21 days of exposure, AA significantly inhibited the hatching rate and delayed the hatching time of embryos, and led to developmental delay, teratogenesis, and locomotion impairments in larvae. RNA-sequencing data of larvae indicated that AA upregulated the expression of hemoglobin and myoglobin involved in oxygen transport and angiopoietin 1, integrin, and matrix metallopeptidases related to angiogenesis and downregulated the expression of early growth response genes and synaptotagmin-2 related to neural plasticity and neurotransmitter release. Overall, our study showed that AA caused deleterious effects on the early development of euryhaline fish through hypoxic stress and neurotoxicity, providing a scientific basis for the environmental risk assessment of marine AA. Highlights: AA reduces hatchability and delays hatching time in marine medaka embryos. AA leads to teratogenesis and locomotion impairments in larvae of marine medaka. RNA-seq indicates that AA induces hypoxia stress and neurotoxicity. AA exhibits the early developmental toxicity on marine fish.