Chitinase involved in immune regulation by mediated the toll pathway of crustacea Procambarus clarkii. Issue 110 (March 2021)

• Record Type: Journal Article
• Authors: Liu, Min; Chen, Chen; Wu, Qi-Cheng; Chen, Jia-Le; Dai, Li-Shang; Hui Chu, Sheng; Liu, Qiu-Ning
• Is Part Of: Fish & shellfish immunology. Issue 110(2021)
• Page Start: 67
• Page End: 74
• Publication Date: 2021-03
• Subjects: Chitinase -- Innate immunity -- PcChitinase 2 gene -- Procambarus clarkii -- Toll pathway; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.12.015
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Chitinase can degrade chitin and play an essential role in animal immunity and plant defense. The immune functions of Chitinase in Procambarus clarkii ( P. clarkii ) remain to elucidate. Here, we identified PcChitinase 2 gene sequence from P. clarkii and studied its spatial and temporal expression profiles. The PcChitinase 2 transcribed unequally in different tissues; however, its expression was highest in those of stomach, gut, and hepatopancreas. The challenge with lipolysaccharide or peptidoglycan significantly up-regulated the expression of PcChitinase 2 in hepatopancreas. The knockdown of the PcChitinase 2 gene by double-stranded RNA suppressed most of the Toll-pathway-related immune genes ( phospholipase, lectin, sptazle Cactus, serine proteikinase, anti-lipopolysaccharide factor, and Toll ) production were significantly increased. Our results suggest PcChitinase 2 may be involved in the innate immune responses of P. clarkii by modulating the toll pathway. Highlights: A Chitinase 2 from Procambarus clarkii ( PcChitinase 2 ) was identified and characterized for the first time. The expression profiles of PcChitinase 2 in various tissues were investigated. Transcripts and expression levels of PcChitinase 2 in the hepatopancreas were detected after LPS and PGN challenge. RNA interference of PcChitinase 2 influenced the expression of immune-related genes.