Juvenile hormone induces methoprene-tolerant 1 phosphorylation to increase interaction with Taiman in Helicoverpa armigera. (March 2021)

• Record Type: Journal Article
• Authors: Li, Yan-Xue; Wang, Di; Zhao, Wen-Li; Zhang, Jun-Ying; Kang, Xin-Le; Li, Yan-Li; Zhao, Xiao-Fan
• Is Part Of: Insect biochemistry and molecular biology. Volume 130(2021)
• Publication Date: 2021-03
• Subjects: Basic-helix-loop-helix Per/Arnt/Sim protein -- Methoprene-tolerant 1 -- Juvenile hormone -- phosphorylation -- Gene transcription; JH juvenile hormone -- Met Methoprene-tolerant -- bHLH-PAS basic-helix-loop-helix Per/Arnt/Sim -- λPPase lambda protein phosphatase -- Kr-h1 Krüppel homolog 1 -- Tai Taiman -- JHRE JH response element -- E-box motif in JHRE -- Thr393 threonine residue at 393 in the protein -- PLC phospholipase C -- PKC protein kinase C -- Phos-tag SDS-PAGE phosphate-affinity sodium dodecyl sulfate–polyacrylamide gel electrophoresis -- 2-DE two-dimensional electrophoresis; Insect biochemistry -- Periodicals; Insects -- Physiology -- Periodicals; Insects -- Molecular aspects -- Periodicals; Biochemistry -- Periodicals; Insectes -- Biochimie -- Périodiques; Insectes -- Composition -- Périodiques; Insectes -- Physiologie -- Périodiques; Insectes -- Aspect moléculaire -- Périodiques; Biochimie -- Périodiques; Biochemistry; Insect biochemistry; Insects -- Molecular aspects; Insects -- Physiology; Periodicals; 572.8157
• DOI: 10.1016/j.ibmb.2021.103519
• Languages: English
• ISSNs: 0965-1748

Abstract

Abstract: Methoprene-tolerant 1 (Met1) is a basic-helix-loop-helix Per/Arnt/Sim (bHLH-PAS) protein identified as the intracellular receptor of juvenile hormone (JH). JH induces phosphorylation of Met1; however, the phosphorylation site and outcomes of phosphorylation are not well characterized. In the present study, using the lepidopteran insect and serious agricultural pest Helicoverpa armigera (cotton bollworm) as a model, we showed that JH III induced threonine-phosphorylation of Met1 at threonine 393 (Thr393) in the Per-Arnt-Sim (PAS) B domain. Thr393-phosphorylation was necessary for Met1 binding to the JH response element (JHRE) to promote the transcription of Kr-h1 (encoding transcription factor Krüppel homolog 1) because Thr393-phosphorylated Met1 increased its interaction with Taiman (Tai) and prevented the Met1-Met1 association. However, JH III could not prevent Met1-Met1 association after Met1-Thr393 was mutated, suggesting that Thr393-phosphorylation is an essential mechanism by which JH prevents Met1-Met1 association. The results showed that JH induces Met1 phosphorylation on Thr393, which prevents Met1-Met1 association, enhances Met1 interaction with Tai, and promotes the binding of Met1-Tai transcription complex to the E-box in the JHRE to regulate Kr-h1 transcription. Graphical abstract: Image 1 Highlights: JH III induces Met1 phosphorylation on Thr393. Met1 phosphorylation on Thr393 enhances its interaction with transcription factor Taiman. Thr393-phosphorylation was necessary for Met1 binding to the JH response element. Met1 phosphorylation on Thr393 prevents Met1-Met1 association.