Three-dimensional molecular concentrator with micrometer-sized constriction for rapid protein enrichment. (29th January 2021)

• Record Type: Journal Article
• Authors: Chang, Yang-Yu; Chen, Chun-Da; Chen, Chien-Chang; Kuo, Ju-Nan
• Is Part Of: Japanese journal of applied physics. Volume 60:Number 2(2021)
• Publication Date: 2021-01-29
• Subjects: electrodeless dielectrophoresis (EDEP) -- constriction structure -- protein enrichment -- streptavidin -- molecular concentrator; Physics -- Periodicals; 621.05
• DOI: 10.35848/1347-4065/abdc34
• Languages: English
• ISSNs: 0021-4922

Abstract

Abstract: A three-dimensional electrodeless dielectrophoresis (EDEP) device incorporating a micrometer-sized constriction region is proposed for the rapid preconcentration of protein. The electrokinetic phenomena within the proposed device are investigated both numerically and experimentally. It is shown that the micro-constriction structure increases the field-focusing factor by around 15 000 times and yields a 10 6 -fold increase in the concentration of streptavidin protein within 36 s. Overall, the proposed device provides a low-cost and effective solution for performing highly-sensitive protein detection and overcomes the Poisson statistical limit inherent in traditional two-dimensional (2D) EDEP devices through its support of large (ml-scale) sample volumes.