Genome editing for plant research and crop improvement. Issue 1 (27th January 2021)

• Record Type: Journal Article
• Authors: Zhan, Xiangqiang; Lu, Yuming; Zhu, Jian‐Kang; Botella, Jose Ramon
• Is Part Of: Journal of integrative plant biology. Volume 63:Issue 1(2021)
• Page Start: 3
• Page End: 33
• Publication Date: 2021-01-27
• Subjects: CRISPR/Cas -- crop improvement -- genome editing -- plant research; Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.13063
• Languages: English
• ISSNs: 1672-9072

Abstract

Abstract: The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a profound impact on plant biology, and crop improvement. In this review, we summarize the state‐of‐the‐art development of CRISPR technologies and their applications in plants, from the initial introduction of random small indel (insertion or deletion) mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting. We describe advances in the use of class 2, types II, V, and VI systems for gene disruption as well as for precise sequence alterations, gene transcription, and epigenome control. Abstract : This review summarizes the state‐of‐the‐art developments in CRISPR technologies and their applications in plant biology and crop improvement, from the initial introduction of random small indel mutations at target genomic loci to precision editing such as base editing, prime editing and gene targeting.