IN VIVO BIODOSIMETRY OF PORCINE T-LYMPHOCYTE SUBSETS AND NK CELLS. Issue 2 (14th January 2020)

• Record Type: Journal Article
• Authors: Andrejsová, Lenka; Šinkorová, Zuzana; Šinkora, Jiří; Tichý, Aleš; Filipová, Alžběta; Němcová, Markéta; Šinkora, Marek
• Is Part Of: Radiation protection dosimetry. Volume 186:Issue 2/3(2019)
• Page Start: 181
• Page End: 185
• Publication Date: 2020-01-14
• Subjects: Radiation dosimetry -- Periodicals; Radiation -- Safety measures -- Periodicals; 363.1799
• DOI: 10.1093/rpd/ncz199
• Languages: English
• ISSNs: 0144-8420

Abstract

Abstract: The aim of the present study was to evaluate the biodosimetric potential of peripheral blood lymphocytes, particularly of T-cell subsets (null and T helper) and natural killer cells (NK), upon exposure to gamma irradiation ( 60 Co) in vivo . For this purpose, the change in relative numbers of NK cells and T-lymphocyte subsets, as well as in the H2AX phosphorylation rate, were evaluated as potential early markers of the lymphocytic response to irradiation in vivo . These experiments were performed on a Large White Pig model. As a result, significant but not dose-dependent changes in the proportion of lymphocyte subpopulations (NK cells, null and T helper cells) were found after exposure to ionising radiation in vivo . On the other hand, circulating NK cells showed relatively higher radioresistance capacity when compared to the T-lymphocyte subsets; however, gamma-H2AX expression showed no significant difference between the evaluated lymphocyte subsets.