Metagenomic analysis for profiling of microbial communities and tolerance in metal-polluted pulp and paper industry wastewater. (March 2021)

• Record Type: Journal Article
• Authors: Sharma, Pooja; Tripathi, Sonam; Chandra, Ram
• Is Part Of: Bioresource technology. Volume 324(2021)
• Publication Date: 2021-03
• Subjects: Microbial community -- Wastewater treatment -- Illumina MiSeq sequencing -- Operational taxonomic unit -- Biological wastewater treatment; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2021.124681
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Graphical representation of metagenomics analysis of pulp and paper industry wastewater via 16S rRNA V3-V4 sequencing. Highlights: Environmental pollutants from industries cause enormous ecological impacts. Bacterial community has strong prospects for removing metal pollutants. The best approach to microbes profiling is use of metagenomic strategies. Abstract: This work aimed to study the profiling and efficiency of microbial communities and their abundance in the pulp and paper industry wastewater, which contained toxic metals, high biological oxygen demands, chemical oxygen demand, and ions contents. Sequence alignment of the 16S rRNA V3-V4 variable region zone with the Illumina MiSeq framework revealed 25356 operating taxonomical units (OTUs) derived from the wastewater sample. The major phyla identified in wastewater were Proteobacteria, Bacteroidetes, Firmicutes, Chloroflexi, Actinobacteria, Spirochetes, Patesibacteria, Acidobacteria, and others including unknown microbes. The study showed the function of microbial communities essential for the oxidation and detoxifying of complex contaminants and design of effective remediation techniques for the re-use of polluted wastewater. Findings demonstrated that the ability of different classes of microbes to adapt and survive in metal-polluted wastewater irrespective of their relative distribution, as well as further attention can be provided to its use in the bioremediation process.