A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: pharmacokinetic outcomes. (26th November 2018)

• Record Type: Journal Article
• Authors: El-Yazbi, Fawzy A; Amin, Omayma A; El-Kimary, Eman I; Khamis, Essam F; Younis, Sameh E; Elkhatib, Mohammed AW; El-Yazbi, Ahmed F
• Is Part Of: Bioanalysis. Volume 11:Number 1(2019)
• Page Start: 41
• Page End: 54
• Publication Date: 2018-11-26
• Subjects: diclofenac -- febuxostat -- HPLC-DAD -- human plasma -- liver uptake -- pharmacokinetics; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2018-0219
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: To develop a simple HPLC-DAD method for simultaneous determination of febuxostat (FEB) and diclofenac (DIC) in biological samples to assess pharmacokinetic outcomes of their coadministration.Methodology & results: Sample preparation was performed by liquid–liquid extraction. Drugs analysis was done on C18 column using methanol-formic acid pH 2.1 (76:24, v/v) as mobile phase and time-programmed UV detection. Lower limits of quantitation for FEB and DIC were 10 and 20 ng/ml, respectively. Baseline pharmacokinetics were similar to published data on either drug alone. Coadministration led to more than twofold increase in FEB Cmax and AUC together with a reduced hepatic uptake in rats.Conclusion: DIC interfered with initial distribution and terminal clearance of FEB potentially due to reduced FEB hepatic uptake.