EARLY BUD BREAK 1 triggers bud break in peach trees by regulating hormone metabolism, the cell cycle, and cell wall modifications. (9th March 2020)

• Record Type: Journal Article
• Authors: Zhao, Xuehui; Han, Xiaolun; Wang, Qingjie; Wang, Xuxu; Chen, Xiude; Li, Ling; Fu, Xiling; Gao, Dongsheng
• Editors: Leubner, Gerhard
• Is Part Of: Journal of experimental botany. Volume 71:Number 12(2020)
• Page Start: 3512
• Page End: 3523
• Publication Date: 2020-03-09
• Subjects: Bud break -- cell cycle -- cell wall modification -- hormone -- peach (Prunus persica) -- PpEBB1 -- PpEXBL1; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/eraa119
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract: In a previous study we identified EARLY BUD BREAK 1 (EBB1), an ERF transcription factor, in peach ( Prunus persica var. nectarina cultivar Zhongyou 4); however, little is known of how PpEBB1 may regulate bud break. To verify the function of PpEBB1 in bud break, PpEBB1 was transiently transformed into peach buds, resulting in early bud break. Bud break occurred earlier in PpEBB1-oe poplar ( Populus trichocarpa ) obtained by heterologous transformation than in wild type (WT), consistent with the peach bud results, indicating that PpEBB1 can promote bud break. To explore how PpEBB1 affects bud break, differentially expressed genes (DEGs) between WT and PpEBB1-oe poplar plants were identified by RNA-sequencing. The expression of DEGs associated with hormone metabolism, cell cycle, and cell wall modifications changed substantially according to qRT-PCR. Auxin, ABA, and total trans -zeatin-type cytokinin levels were higher in the PpEBB1-oe plants than in WT plants, while the total N 6 -(Δ 2 -isopentenyl)-adenine-type cytokinins was lower. Yeast two-hybrid and bimolecular fluorescence complementation assays verified that a cell wall modification-related protein (PpEXBL1) interacted with PpEBB1 suggesting that PpEBB1 could interact with these cell wall modification proteins directly. Overall, our study proposed a multifaceted explanation for how PpEBB1 regulates bud break and showed that PpEBB1 promotes bud break by regulating hormone metabolism, the cell cycle, and cell wall modifications. Abstract : The transcription factor EBB1 plays important roles in regulating bud break in peach trees by regulating hormone metabolism, the cell cycle, and cell wall modifications.