Nanocomposite Bienzymatic Sensor for Monitoring Xanthine in Wound Diagnostics. Issue 9 (10th June 2019)

• Record Type: Journal Article
• Authors: RoyChoudhury, Sohini; Umasankar, Yogeswaran; Bhushan, Pulak; Hirt, Penelope A.; MacQuhae, Flor E.; Borda, Luis J.; Lev-Tov, Hadar A.; Kirsner, Robert; Bhansali, Shekhar
• Is Part Of: Journal of the Electrochemical Society. Volume 166:Issue 9(2019)
• Page Start: B3295
• Page End: B3301
• Publication Date: 2019-06-10
• Subjects: Bioelectrochemistry -- Electrochemical Engineering -- Sensors -- electrochemical biosensor -- Wound healing -- xanthine detection; Electrochemistry -- Periodicals; 541.3705
• DOI: 10.1149/2.0401909jes
• Languages: English
• ISSNs: 0013-4651

Abstract

Abstract : This work reports a biosensor for monitoring xanthine for potential wound healing assessment. Active substrate of the biosensor has xanthine oxidase (XO) and horseradish peroxidase (HRP) physisorbed on a nanocomposite of multiwalled carbon nanotubes (MWCNT) decorated with gold nanoparticles (AuNP). The presence of HRP provided a two-fold increase in response to xanthine, and a three-fold increase in response to the nanocomposite. With a sensitivity of 155.71 nA μM −1 cm −2 the biosensor offers a detection limit of 1.3 μM, with linear response between 22 μM and 0.4 mM. Clinical sample analyses showed the feasibility of xanthine detection from biofluids in a lesion site due to diffusion of the analyte into surrounding biofluids. Higher concentrations by three-fold were observed from wound proximity, than away from injury, with an average recovery of 110%. Results show the feasibility of monitoring wound severity through longitudinal measurements of xanthine from injured vicinity.