A sensitive method for the quantitation of the peptide-based glucagon-like peptide-1 receptor agonist liraglutide in plasma using microfluidics chromatography tandem MS. (5th March 2018)

• Record Type: Journal Article
• Authors: King-Ahmad, Amanda J; Kalgutkar, Amit S; Niosi, Mark; Eng, Heather; Holliman, Christopher
• Is Part Of: Bioanalysis. Volume 10:Number 5(2018)
• Page Start: 357
• Page End: 368
• Publication Date: 2018-03-05
• Subjects: agonist -- bioanalytical -- ELISA -- enzyme-linked immunosorbent assay -- GLP-1 -- glucagon-like peptide-1 -- LC–MS/MS -- liquid chromatography tandem mass Spectrometry -- liraglutide -- monkey -- pharmacokinetics -- plasma -- receptor; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2017-0239
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: An LC–MS/MS assay for the quantitation of liraglutide, a peptide-based injectable glucagon-like peptide-1 receptor agonist, has been developed as a convenient alternative to the enzyme-linked immunosorbent assay, and used to characterize liraglutide pharmacokinetics in cynomolgus monkeys.Results: Assay calibration curves exhibited a linear dynamic range of 10–5000 ng/ml and correlation coefficient ≥0.98. Following a 30 μg/kg intravenous dose, liraglutide demonstrated low plasma clearance and distribution volume, which led to a terminal half-life of 6.59 h in monkeys.Conclusion: The dynamic range of our LC–MS/MS assay provides sufficient coverage of the average efficacious liraglutide concentrations in human plasma, and can be used for pharmacokinetics/pharmacodynamics studies in animals and potentially in humans.