A sensitive and selective LC–MS/MS method for quantitation of ivermectin in human, mouse and monkey plasma: clinical validation. (16th October 2018)

• Record Type: Journal Article
• Authors: Chhonker, Yashpal S; Ma, Liping; Edi, Constant; Murry, Daryl J
• Is Part Of: Bioanalysis. Volume 10:Number 22(2018)
• Page Start: 1841
• Page End: 1852
• Publication Date: 2018-10-16
• Subjects: abamectin -- ivermectin -- LC–MS/MS -- pharmacokinetics; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2018-0110
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: A sensitive and selective LC–MS/MS method was validated for quantitation of ivermectin (IVM) in plasma.Method: The IVM was extracted from plasma using solid-phase extraction with C-18 cartridges. Separation of analytes was achieved on an ACE C18 column with isocratic elution using 0.1% acetic acid and methanol: acetonitrile (1:1, v/v) as mobile phase. The IVM was quantitated using electrospray ionization operating in negative multiple reaction monitoring mode.Results: The MS/MS response was linear over the concentration range from 0.1–1000 ng/ml. The method for human plasma was validated as per US FDA guidelines. The LC–MS/MS method is sensitive, reproducible, has easy sample preparation and is suitable for IVM quantitation in clinical samples.