Plasmid DNA-loaded asymmetrically porous membrane for guided bone regeneration. (10th February 2021)

• Record Type: Journal Article
• Authors: Oh, Se Heang; Byun, June-Ho; Chun, So Young; Jang, Young-Joo; Lee, Jin Ho
• Is Part Of: Journal of materials science & technology. Volume 63(2021)
• Page Start: 161
• Page End: 171
• Publication Date: 2021-02-10
• Subjects: Plasmid DNA (pDNA) -- Guided bone regeneration (GBR) -- Bone morphogenetic protein-2 (BMP-2) -- Polycaprolactone (PCL) -- Gene delivery; Metals -- Periodicals; Materials science -- Periodicals; Materials science; Metals; Periodicals; 620.1105
• DOI: 10.1016/j.jmst.2020.03.015
• Languages: English
• ISSNs: 1005-0302

Abstract

Abstract: Although bone defects can be restored spontaneously, bone reconstruction with sufficient strength and volume continues to be a challenge in clinical practices. In recent years, the use of a variety of biomaterials with bioactivity has been attempted to compensate for this limitation. Herein, we fabricated a pDNA (encoding for BMP-2)-loaded asymmetrically porous polycaprolactone (PCL)/Pluronic F127 membrane as a bioactive guided bone regeneration (GBR) membrane, using a modified immersion-precipitation method. It was observed that the GBR membrane allows continuous release of pDNA for more than 20 weeks. The pDNA was sufficiently transfected into human bone marrow stem cells ( h BMSCs) without significant cytotoxicity and the gene-transfected cells showed prolonged synthesis of BMP-2. From in vitro osteogenic differentiation and in vivo animal studies, the effective induction of osteogenic differentiation of h BMSCs and enhanced bone regeneration by the pDNA-loaded asymmetrically porous PCL/Pluronic F127 membrane was observed, suggesting that the pDNA-loaded membrane as a bioactive GBR membrane can be an alternative therapeutic technique for effective bone regeneration.