Validation of direct method to quantify dexamethasone in human aqueous humor by LC–MS/MS. (4th September 2018)

• Record Type: Journal Article
• Authors: Ferreira, Mônica Siqueira; Marquez, Cláudio Roberto; dos Santos, Danieli Almeida; Gabbai, José Jorge; Martho, Aline Cristina; Yamanouchi Brandão, Amanda Hayashi; Barella, Kleyton Arlindo; Riccio, Maria Francesca; Noboli, Ana Cláudia; Júnior, Pedro Serafim
• Is Part Of: Bioanalysis. Volume 10:Number 17(2018)
• Page Start: 1361
• Page End: 1370
• Publication Date: 2018-09-04
• Subjects: aqueous humor -- dexamethasone -- direct method -- LC–MS/MS -- small volume -- validation; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2018-0079
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: Dexamethasone (Dex) has been used for the treatment of ocular diseases, presenting concentrations up to 150 ng/ml in the biological matrix. Drug sampling has been performed from aqueous humor (AH), which the volume varies from 50 to 200 μl, becoming a challenge for analytical analyses.Results: We developed and validated a direct and sensitive method by LC–MS/MS for Dex measurement in human AH, using water as surrogate matrix to reduce the sample volume applied in some validation assays. With accuracies of 99.6% and precision within 15% in the range of 0.1–150 ng/ml, Dex had an 87-day stability.Conclusion: Our method is robust and sensitive enough to be applied in bioequivalence studies with human AH using only 20 μl of biological sample.