Proposed selection strategy of surrogate matrix to quantify endogenous substances by Japan Bioanalysis Forum DG2015-15. (4th September 2018)

• Record Type: Journal Article
• Authors: Wakamatsu, Akira; Ochiai, Shoko; Suzuki, Eiko; Yokota, Yoshinobu; Ochiai, Midori; Kotani, Yosuke; Sasahara, Satomi; Nakanaga, Keita; Hashimoto, Yuki; Ueno, Satoko; Kato, Nozomu; Kawada, Satoshi; Hayakawa, Jun; Shimada, Eiichi; Horita, Shinya; Sakai, Kazuaki
• Is Part Of: Bioanalysis. Volume 10:Number 17(2018)
• Page Start: 1349
• Page End: 1360
• Publication Date: 2018-09-04
• Subjects: BMV guideline -- endogenous substance -- evaluation of appropriateness -- Japan Bioanalysis Forum -- LBA -- LC–MS -- quantitative analysis -- recommended method -- selection -- surrogate matrix; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2018-0105
• Languages: English
• ISSNs: 1757-6180

Abstract

It is important to select an appropriate surrogate matrix for preparing calibration standards and quality control samples while quantitatively assaying for endogenous substances, because a blank matrix that does not contain the endogenous substance cannot be derived from the species from which the target study samples are collected. This is because the assay results might be affected, depending on the characteristics of the analyte in the surrogate matrix. Our discussion group that participated in the Japan Bioanalysis Forum discussed the recommended selection strategies, focusing on large and small molecules in ligand binding assays and LC–MS, respectively. We established an efficient selection strategy for a surrogate matrix, with simple compositions as the first candidates stated in this article.