Inhibition of osteoblast proliferation and migration by exogenous and endogenous formaldehyde. (May 2021)

• Record Type: Journal Article
• Authors: Teng, Xu; Wang, Pei; Yang, Tianshu; Huang, Wei; Yu, Hefeng; Li, Weishi; Chen, Zhongqiang; Fan, Dongwei
• Is Part Of: Human & experimental toxicology. Volume 40:Number 5(2021)
• Page Start: 882
• Page End: 894
• Publication Date: 2021-05
• Subjects: Formaldehyde -- cell growth -- cell migration -- RNA-seq -- foxo1; Toxicology -- Periodicals; 615.9
• DOI: 10.1177/0960327120975125
• Languages: English
• ISSNs: 0960-3271

Abstract

Exogenous and endogenous formaldehyde (FA) both play an important role in cell growth and migration; however, their potential role in osteoblasts remains largely unclear. Cell counting kit-8 (CCK-8) and wound-healing assays revealed that FA exposure at naturally occurring concentrations inhibited the proliferation and migration of mouse preosteoblast MC3T3-E1 cells. Moreover, RNA sequencing (RNA-seq) analysis revealed that FoxO1 signaling pathway components displayed distinct expression patterns upon FA exposure, reflected through significant enrichment of cell migration. In particular, FoxO1-, Sirt1-, and FA-induced protein expression, which was closely associated with cell proliferation and migration, was confirmed by western blotting. The results obtained indicated that the FoxO1 pathway is involved in FA-induced inhibition of cell growth and migration.