Polyketides from the marine-derived fungus Aspergillus falconensis: In silico and in vitro cytotoxicity studies. (1st January 2021)

• Record Type: Journal Article
• Authors: El-Kashef, Dina H.; Youssef, Fadia S.; Reimche, Irene; Teusch, Nicole; Müller, Werner E.G.; Lin, Wenhan; Frank, Marian; Liu, Zhen; Proksch, Peter
• Is Part Of: Bioorganic & medicinal chemistry. Volume 29(2021)
• Publication Date: 2021-01-01
• Subjects: Aspergillus falconensis -- OSMAC -- Molecular docking -- Cytotoxicity -- Polyketides; Bioorganic chemistry -- Periodicals; Pharmaceutical chemistry -- Periodicals; Biochemistry -- Periodicals; Chemistry, Clinical -- Periodicals; Chemistry, Organic -- Periodicals; Chimie bio-organique -- Périodiques; Chimie pharmaceutique -- Périodiques; 615.19
• DOI: 10.1016/j.bmc.2020.115883
• Languages: English
• ISSNs: 0968-0896

Abstract

Graphical abstract: Abstract: Fermentation of the marine-derived fungus Aspergillus falconensis, isolated from sediment collected from the Red Sea, Egypt on solid rice medium containing 3.5% NaCl yielded a new dibenzoxepin derivative (1 ) and a new natural isocoumarin (2 ) along with six known compounds (3 –8 ). Changes in the metabolic profile of the fungus were induced by replacing NaCl with 3.5% (NH4 )2 SO4 that resulted in the accumulation of three further known compounds (9 –11 ), which were not detected when the fungus was cultivated in the presence of NaCl. The structures of the new compounds were elucidated by HRESIMS and 1D/2D NMR as well as by comparison with the literature. Molecular docking was conducted for all isolated compounds on crucial enzymes involved in the formation, progression and metastasis of cancer which included human cyclin-dependent kinase 2 (CDK-2), human DNA topoisomerase II (TOP-2) and matrix metalloproteinase 13 (MMP-13). Diorcinol (7 ), sulochrin (9 ) and monochlorosulochrin (10 ) displayed notable stability within the active pocket of CDK-2 with free binding energy (ΔG) equals to −25.72, −25.03 and −25.37 Kcal/mol, respectively whereas sulochrin (9 ) exerted the highest fitting score within MMP-13 active center (ΔG = −33.83 Kcal/mol). In vitro cytotoxic assessment using MTT assay showed that sulochrin (9 ) exhibited cytotoxic activity versus L5178Y mouse lymphoma cells with an IC50 value of 5.1 µM and inhibition of migration of MDA-MB 231 breast cancer cells at a concentration of 70 µM.