A label-free platform for dopamine biosensing. (January 2018)

• Record Type: Journal Article
• Authors: Navarro, Jesús; Galbán, Javier; Marcos, Susana de
• Is Part Of: Bioanalysis. Volume 10:Number 1(2018)
• Page Start: 11
• Page End: 21
• Publication Date: 2018-01
• Subjects: dopamine -- laccase -- optical biosensor; Drugs -- Analysis -- Periodicals; 615.19005
• DOI: 10.4155/bio-2017-0161
• Languages: English
• ISSNs: 1757-6180

Abstract

Aim: This work presents a label-free platform for dopamine (DA) monitoring based on the spectroscopic properties of laccase.Results: Working in batch mode, DA ranging from 25 to 250 μM, can be determined without the interference of norepinephrine and epinephrine. Laccase immobilized in a polyacrylamide film is the basis of a platform for the label-free determination of DA. The linear range goes from 100 to 900 μM with an RSD of 5.3% and a film lifetime of more than 30 measurements. The biosensors also permit the DA + epinephrine + norepinephrine determination.Conclusion: The method permits the determination of DA and the total concentration of the three neurotransmitters, and could be used for DA monitoring in urine samples.