Effect of composting on the conjugative transmission of sulfonamide resistance and sulfonamide-resistant bacterial population. (20th February 2021)

• Record Type: Journal Article
• Authors: Lin, Hui; Sun, Wanchun; Jin, Danfeng; Yu, Qiaogang; Yang, Yuyi; Zhang, Zulin; Sun, Pengfei; Ma, Junwei
• Is Part Of: Journal of cleaner production. Volume 285(2021)
• Publication Date: 2021-02-20
• Subjects: Antibiotic resistance gene -- Bacterial conjugation -- Bacterial pathogen -- Horizontal gene transfer -- Bacillus anthracis; Factory and trade waste -- Management -- Periodicals; Manufactures -- Environmental aspects -- Periodicals; Déchets industriels -- Gestion -- Périodiques; Usines -- Aspect de l'environnement -- Périodiques; 628.5
• DOI: 10.1016/j.jclepro.2020.125483
• Languages: English
• ISSNs: 0959-6526

Abstract

Abstract: The effect of composting practice on reducing the health risk of manure-derived antibiotic resistance is mainly assessed based on the abundance of antibiotic resistance determinants. This study evaluated the impact of industrial thermophilic manure composting in controlling the risk of sulfonamide resistance (SR) by identifying the patterns of horizontal gene transfer events via conjugation experiments and analyzing the community distribution of sulfonamide-selectively cultured bacteria (SRB) via 16S rRNA sequencing. Results showed that composting removed 75.9% of sulfonamide residues and inhibited the transfer of SR from manure to an Escherichia coli recipient, with variation according to the resistance gene. Composting decreased the abundances and conjugative transfer of sul2 and intI1 effectively but not that of sul1, which is essential for E. coli SR acquisition, and it was more effective in suppressing the co-transfer of sul1 with sul2 / intI1 rather than the transfer of sul1 individually. Besides, it was found that SRB community responded more effectively to composting when compared with the total bacterial community. The SRB in raw compost showed a predominance of Proteobacteria, whereas the SRB in mature compost mirrored the total bacteria community. Bacillus anthracis, a major sulfonamide-resistant pathogen, was enriched after composting. Overall, composting failed to reduce the risks of sul1 and sulfonamide-resistant pathogenic bacteria effectively, which should be essential targets to optimize manure composting for decreasing the dissemination of antibiotic resistance. Graphical abstract: Image 1 Highlights: Composting affects sulfonamide resistance transfer potential and pattern in manure. Composting is more prone to decrease co-transfer of sul1 and sul2 / intI1 . Raw and mature composts differ in sulfonamide-resistant bacterial population. Composting causes enrichment of sulfonamide-resistant pathogenic bacteria. The resistance risk related to sul1 and Bacillus anthracis persists in compost.