Effective fluorescence‐guided surgery of liver metastasis using a fluorescent anti‐CEA antibody. Issue 8 (3rd October 2016)

• Record Type: Journal Article
• Authors: Hiroshima, Yukihiko; Lwin, Thinzar M.; Murakami, Takashi; Mawy, Ali A.; Kuniya, Tanaka; Chishima, Takashi; Endo, Itaru; Clary, Bryan M.; Hoffman, Robert M.; Bouvet, Michael
• Is Part Of: Journal of surgical oncology. Volume 114:Issue 8(2016)
• Page Start: 951
• Page End: 958
• Publication Date: 2016-10-03
• Subjects: fluorescence‐guided surgery -- colon‐cancer liver metastasis -- orthotopic‐liver metastasis model -- fluorescent anti‐CEA antibody -- survival -- disease‐free survival; Cancer -- Surgery -- Periodicals; Neoplasms -- Periodicals; 616
• DOI: 10.1002/jso.24462
• Languages: English
• ISSNs: 0022-4790

Abstract

Abstract : Background and Objectives: Delineation of adequate tumor margins is critical in oncologic surgery, particularly in resection of metastatic lesions. Surgeons are limited in visualization with bright‐light surgery, but fluorescence‐guided surgery (FGS) has been efficacious in helping the surgeon achieve negative margins. Methods: The present study uses FGS in a mouse model that has undergone surgical orthotopic implantation (SOI) of colorectal liver metastasis tagged with green fluorescent protein (GFP). An anti‐CEA antibody conjugated to DyLight 650 was used to highlight the tumor. Results: The fluorescent antibody clearly demarcated the lesion at deeper tissue depth compared to GFP. Fluorescence of the anti‐CEA‐DyLight650 showed maximal tumor‐to‐liver contrast at 72 hr. Fifteen mice underwent bright‐light surgery (BLS) versus FGS with GFP versus FGS with anti‐CEA‐DyLight650. Mice that underwent FGS had a significantly smaller area of residual tumor ( P < 0.001) and significantly longer overall survival ( P < 0.001) and disease‐free survival ( P < 0.001). Within the two FGS groups, mice undergoing surgery with anti‐CEA‐DyLight650 improved survival compared to only GFP labeling. Conclusions: In the present report, we demonstrate that an anti‐CEA antibody conjugated to a DyLight 650 nm dye clearly labeled colon cancer liver metastases, thereby enabling successful FGS. J. Surg. Oncol. 2016;114:951–958 . © 2016 Wiley Periodicals, Inc.