Validation of the RIDASCREEN®FAST Milk Kit. (27th November 2019)

• Record Type: Journal Article
• Authors: Weiss, Thomas; Lacorn, Markus; Flannery, Jonathan; Benzinger, M Joseph; Bird, Patrick; Crowley, Erin S; Goins, Dave; Agin, James R; Gilani, Sarwar; Poepping, Bert; Garber, Eric
• Is Part Of: Journal of AOAC International. Volume 99:Number 2(2016)
• Page Start: 495
• Page End: 503
• Publication Date: 2019-11-27
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.15-0290
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: The RIDASCREEN ® FAST Milk test is a sandwich ELISA for the rapid quantification of milk proteins in various foods. The specific antibodies target casein and β-lactoglobulin. Samples are extracted and can then be analyzed in less than 40 min. The calibration curve covers a range from 2.5 to 67.5 mg/kg milk protein. The assay was validated with cookies, infant formula, chocolate dessert, ice cream, and sausages. All negative samples were found well below the LOQ of 2.5 mg/kg. Recoveries of the spiked samples were mostly in the range of 80–120%. The LOD of the ELISA was found below 1 mg/kg. The analysis of 39 different substances of interest revealed that no cross-reactivity above the LOQ occurred. Ruggedness testing proved that variations in incubation temperature, reagent volume, incubation time, extraction temperature, and extraction time had no significant influence. The stability at 4–8°C of three independent lots was investigated and found to exceed 18 months. Very good lot-to-lot consistency and no significant loss of the analytical capacity over the shelf life were observed. Incurred cookies and chocolate dessert samples were prepared and analyzed by an independent laboratory; mean recoveries of 94.4 and 102.2% and mean SDs of 10.9 and 6.3%, respectively, were found. For the 0 mg/kg level for both materials, all samples tested returned values of <2.5 mg/kg. Therefore, the analytical performance claims of the manufacturer were confirmed.