Detoxification of Deoxynivalenol by 60Co γ-ray Irradiation and Toxicity Analyses of Radiolysis Products. (3rd December 2019)

• Record Type: Journal Article
• Authors: Li, Mengmeng; Guan, Erqi; Bian, Ke
• Is Part Of: Journal of AOAC International. Volume 102:Number 6(2019)
• Page Start: 1749
• Page End: 1755
• Publication Date: 2019-12-03
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.1093/jaoac/102.6.1749
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: Background: Deoxynivalenol (DON) is a type B trichothecene that occurs predominantly in grains such as wheat, maize, and barley and has been implicated in incidents of mycotoxicoses in both humans and farm animals. Objective: In the present study, we chose 60 Co γ-ray irradiation to degrade DON. Methods: First, the degradation effect of irradiation on DON was analyzed. Second, the toxicity analyses of radiolysis products were studied by oral gavage. Results: The results indicated that 60 Co γ-ray irradiation had significant degradation effect on pure DON: when 20 kGy γ-ray irradiation was used for 2 μg/mL DON in acetonitrile–water, the degradation efficiency of DON was 83%, and 2 μg/mL DON in ultra-pure water was completely degraded after 5 kGy γ-ray irradiation. The concentration of 200 μg/mL DON in ultra-pure water had significant toxicity to mice: decreased body weight gain and feed consumption as well as pathological changes in liver and kidney were observed compared with the control group. Conclusions: No significant toxicity was observed in mice that were given these degraded solutions treated by γ-ray irradiation, which indicated that the toxicity of radiolysis products in ultra-pure water had significantly decreased after treatment by γ-ray irradiation. Highlights: This research offered some reference to detoxify DON in cereal grains.