Determination of Triazines and Triazoles in Grapes Using Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization High-Resolution Mass Spectrometry. (22nd November 2019)

• Record Type: Journal Article
• Authors: Mahale, Vishal; Singh, Ajeet; Phadke, Gayatri S; Ghanate, Avinash D; Oulkar, Dasharath P; Banerjee, Kaushik; Panchagnula, Venkateswarlu
• Is Part Of: Journal of AOAC International. Volume 100:Number 3(2017)
• Page Start: 640
• Page End: 646
• Publication Date: 2019-11-22
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.17-0047
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: A chromatography-free atmospheric pressure matrix-assisted laser desorption/ionization high-resolution massspectrometry (AP-MALDI HRMS) method is described for the simultaneous and quantitative detection of triazines and triazoles in grapes. The analytes were detected reproducibly with high mass accuracy (mass error within 5 ppm) and further confirmed by collision-induced dissociation fragmentation in tandem MS. The LODs and LOQs forall the analytes were found to be in the nanogram per gram level (15–20 ng/g LOQ). Internal standard–normalized high-resolution accurate mass–extracted (HR-AM) peak intensities of the detected ions were used to generate the concentration response curves. Linearity (with R 2 values around 0.99) was obtained for these curves within a concentration range of 20–200 ng/g of the individual analytes. The accuracy and precision of the method were further established using QC samples. Validation and performancecomparison of the AP-MALDI HRMS method with an existing standard method using LC with triple quadrupole MS was carried out (evaluating sensitivity, accuracy, precision, and analysis time) using 20 table-grape field samples after QuEChERS extraction.