Simultaneous Determination of 15 Flavonoids in Scutellaria barbata–Hedyotis diffusa Herb Pair by HPLC Q-TOF MS. (23rd November 2019)

• Record Type: Journal Article
• Authors: Yang, Wei; He, Shiyong; Xiao, Nan; Qiao, Yanling; Sui, Hua; Liang, Lina; Chen, Jing; Li, Weiling; Zhang, Lin
• Is Part Of: Journal of AOAC International. Volume 102:Number 1(2019)
• Page Start: 75
• Page End: 80
• Publication Date: 2019-11-23
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.17-0469
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: Background: The Scutellaria barbata – Hedyotis diffusa herb pair has appeared in classical prescriptions and has usually been used for the treatment of cancer and inflammatory conditions. Objective: For quality control of this herb pair, a rapid and reliable HPLC-quadrupole-time-of-flight(Q-TOF)-MS method was used for the simultaneous determination of alpinetin, apigenin-7-O-β-D-glucopyranoside, scutellarein, apigenin, quercetin-3-O-β-D-glucopyranoside, wogonoside, quercetin, amentoflavone, wogonin, chrysin, luteolin, rutin, naringenin, baicalein, and baicalin in the S. barbata–H. diffusa herb pair. Methods: LC-MS analysis was performed on a Shimadzu HPLC 20ADXR LC system in-line with an AB-SCIEX 5600 Triple TOF mass spectrometer. Results: The calibration curves showed good linearity in a relatively wide concentration range ( r >0.996). The LOD and LOQ were less than 20 and 68 ng/mL, respectively. The relative standard deviation (RSD) values of intraday and inter-day precision were <3.31% for all the analytes. The recoveries of the method were in the range of 94.6–106.0%, with RSD <4.72%. The RSD values of peak areas in the stability study were not more than 4.28% for all analytes within 2 days. These results revealed that the method exhibited good sensitivity, precision, accuracy, and stability. Conclusions: This method could be applied to the quantitative determination of 15 flavonoids in the S. barbata – H. diffusa herb pair. Highlights: ( 1 ) The established method of HPLC-Q-TOF-MS is simple, fast, sensitive, and reliable. ( 2 ) 15 Flavonoids in the S. barbata–H. diffusa herb pair were simultaneous determined.