Establishment of a Decaplex PCR-Capillary Gel Electrophoresis Method for the Simultaneous Detection of Six Kinds of Genetically Modified Animals. (23rd November 2019)

• Record Type: Journal Article
• Authors: Liu, Xiaofei; Qiu, Songyin; Li, Xiaolin; Liu, Dandan; Jing, Hongli; Wang, Qin; Lin, Xiangmei; Pan, Dengke; Shi, Ningning
• Is Part Of: Journal of AOAC International. Volume 101:Number 2(2018)
• Page Start: 601
• Page End: 606
• Publication Date: 2019-11-23
• Subjects: Agricultural chemistry -- Periodicals; Food -- Analysis -- Periodicals; 543
• DOI: 10.5740/jaoacint.17-0201
• Languages: English
• ISSNs: 1060-3271

Abstract

Abstract: This study aimed to establish an event-specific multiplex PCR system using microsatellite markers and fluorescently labeled primers to detect six different genetically modified (GM) animal lines, including human lactoferrin GM cattle, human lysozyme GM cattle, human α-lactalbumin GM cattle, myostatin knockout pigs, phytase GM pigs, and ω-3 fatty acid desaturase gene GM pigs. Four different microsatellite loci for species identification, along with six GM animal-specific fragments, were selected as targets for primer design. The capillary gel electrophoresis results of multiplex PCR showed that the target fragments were amplified successfully. This high-throughput multiplex PCR detection system can be applied for the inspection and quarantine of GM animals.