Comment on 'A novel method for fast and robust estimation of fluorescence decay dynamics using constrained least-square deconvolution with Laguerre expansion'. (1st February 2017)

• Record Type: Journal Article
• Authors: Zhang, Yongliang; Li, David Day-Uei
• Is Part Of: Physics in medicine & biology. Volume 62:Number 4(2017:Feb.)
• Page Start: 1632
• Page End: 1636
• Publication Date: 2017-02-01
• Subjects: Gaussian noise -- Poisson noise -- FLIM -- Laguerre expansion -- deconvolution; Biophysics -- Periodicals; Medical physics -- Periodicals; 610.153
• DOI: 10.1088/1361-6560/aa522f
• Languages: English
• ISSNs: 0031-9155

Abstract

Abstract: This comment is to clarify that Poisson noise instead of Gaussian noise shall be included to assess the performances of least-squares deconvolution with Laguerre expansion (LSD-LE) for analysing fluorescence lifetime imaging data obtained from time-resolved systems. Moreover, we also corrected an equation in the paper. As the LSD-LE method is rapid and has the potential to be widely applied not only for diagnostic but for wider bioimaging applications, it is desirable to have precise noise models and equations.