Characterizing spatiotemporal progression and prediction of infarct lesion volumes in experimental acute ischemia using quantitative perfusion and diffusion imaging. (February 2021)

• Record Type: Journal Article
• Authors: Huang, Hai-Tao; Tung, Tao-Hsin; Lin, Min; Wang, Xinmin; Li, Xie; Liang, Kaimin; Qian, Qi; Chen, Pei-En
• Is Part Of: Applied radiation and isotopes. Volume 168(2021)
• Publication Date: 2021-02
• Subjects: Acute ischemic stroke -- Quantitative perfusion -- Diffusion imaging; Radiology -- Periodicals; Radiation -- Industrial applications -- Periodicals; Nuclear chemistry -- Periodicals; Internet resource; Periodical; 660.298
• DOI: 10.1016/j.apradiso.2020.109522
• Languages: English
• ISSNs: 0969-8043

Abstract

Abstract: Purpose: This study was conducted to explore the diagnostic value of arterial spin labeling (ASL) combined with diffusion weighted imaging (DWI) in characterizing the spatiotemporal progression of infarct lesions in a rabbit middle cerebral artery occlusion (MCAO) model and predicting the acute cerebral infarction (ACI) volume. Materials and methods: Forty-two male rabbits (2.9 ± 0.2 kg body weight) were used in this experimental study. Animals were initially anesthetized by intravenous injection of uratan. There were seven experimental groups with six rabbits in each group. The apparent diffusion coefficient (ADC) and cerebral blood flow (CBF) thresholds were established in the control group (n = 6), which were sacrificed at 12 h, stained for infarct volume, and imaged at each time point. Results: The normal ADC and CBF were estimated as 0.90 ± 0.03 × 10 −3 mm 2 /s and 0.68 ± 0.06 mL g −1 min −1, respectively. The viability thresholds of ADC and CBF yielding the lesion volumes (LVs) at 3 h, which best approximated the 2, 3, 5-triphenyltetrazolium chloride (TTC) infarct volumes at 12 h, were 0.52 ± 0.02 × 10 −3 mm 2 /s (42.2 ± 3% reduction) and 0.33 ± 0.09 mL g −1 min −1 (51.0 ± 11% reduction), respectively. The temporal evolution of the ADC- and CBF-defined LVs showed a significant perfusion/diffusion mismatch up to 1 h (p = 0.001). Conclusion: ADC values and ACI volumes were positively correlated, while CBF was negatively correlated, which is supposed to be a reference for predicting ACI volume. Highlights: To follow the evolution of the diffusion/perfusion mismatch in a New Zealand white rabbit acute autologous thrombosis MCAO model and to predict the ACI volume. The survival thresholds of CBF and ADC in the permanent MCAO model of rabbits were established by using quantitative perfusion and diffusion imaging, and reliability tests were performed independently. By using these survival thresholds to analyze the ischemic process, the diffusion/perfusion mismatch could be identified. The value of ADC was positively correlated with the infarct volume, while the value of CBF was negatively correlated with the infarct volume. Characterizing spatiotemporal progression and prediction of infarct lesion volumes.