Bioprocessing of Chaetoceros calcitrans for the recovery of fucoxanthin using CO2-based alkyl carbamate ionic liquids. (February 2021)

• Record Type: Journal Article
• Authors: Khoo, Kuan Shiong; Ooi, Chien Wei; Chew, Kit Wayne; Foo, Su Chern; Show, Pau Loke
• Is Part Of: Bioresource technology. Volume 322(2021)
• Publication Date: 2021-02
• Subjects: Chaetoceros calcitrans -- Fucoxanthin -- Carotenoids -- Extraction -- Ionic liquids -- CO2; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2020.124520
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Fucoxanthin was extracted successfully from Chaetoceros calcitrans microalgae. Readily distillable CO2 -based carbamate ILs used in the extraction of fucoxanthin. Extracted fucoxanthin exhibits good antioxidant activity in TEAC and TPC analyses. Optimized DACARB system showed excellent recyclability and extraction performance. Abstract: Ionic liquids (ILs) have emerged as an alternative solvent used in the bioprocessing of microalgae for recovery of valuable biomolecules. The aim of this work is to extract fucoxanthin from Chaetoceros calcitrants ( C. calcitrans ) by using the readily distillable CO2 -based alkyl carbamate ILs. The degree of cell permeabilization was analysed by the quantification of extracted fucoxanthin and the analyses of cell surface morphology. Among the tested CO2 -based alkyl carbamate ILs, diallylammonium diallylcarbamate (DACARB) extraction system gave the maximal yield of fucoxanthin at 17.51 mg/g under the optimal extraction conditions [90% (v/v), 3 min and 25 °C]. Moreover, the extracted fucoxanthin fraction exhibited the satisfactory antioxidant activities. The recyclability of DACARB was demonstrated in the multiple batches of fucoxanthin extraction. Hence, CO2 -based alkyl carbamate ILs can prospectively substitute conventional organic solvents in the downstream processing of bioactive compounds from microalgae.