Effect of endurance exercise duration on muscle hypertrophy induced by functional overload. Issue 1 (8th December 2020)

• Record Type: Journal Article
• Authors: Shirai, Takanaga; Obara, Tsubasa; Takemasa, Tohru
• Is Part Of: FEBS open bio. Volume 11:Issue 1(2021)
• Page Start: 85
• Page End: 94
• Publication Date: 2020-12-08
• Subjects: endurance training -- exercise duration -- hypertrophy -- mTOR signaling -- protein breakdown -- resistance training; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.13028
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : For many ball games, both resistance and endurance training are necessary to improve muscle strength and endurance capacity. Endurance training has been reported to inhibit muscle strength and hypertrophy, but some studies have reported that endurance exercise (EE) does not inhibit the effects of resistance exercise. Here, we examined the effect of short‐ or long‐duration EE on mouse skeletal muscle hypertrophy induced by functional overload (OL) at the molecular level. Plantaris muscle hypertrophy was induced by OL with synergist ablation in mice. Body mass was reduced with endurance training, but EE duration (30 or 90 min) had no effect. The ratio of plantaris muscle weight to body weight was higher in the OL and EE for 30 min (OL+EE30) and OL and EE for 90 min (OL+EE90) groups compared with the OL group. Expression of mechanistic target of rapamycin signaling proteins, which is related to protein synthesis and hypertrophy, was increased in the OL+EE30 group. Expression of Forkhead box‐containing protein O1, which is related to protein breakdown and atrophy, remained unchanged. However, microtubule‐associated protein 1 light chain 3, a known marker of autophagy, and MAFbx, which is related to protein breakdown, were significantly increased in the OL+EE90 group. Furthermore, markers of oxidative stress, ubiquitin and 4‐hydroxynonenal were also significantly increased in the OL+EE90 group compared with other groups. In conclusion, EE duration did not affect body mass and plantaris mass and did not interfere with mechanistic target of rapamycin signaling, but it did increase ubiquitinated proteins and oxidative stress. It is therefore necessary to consider training durations for EE when combining endurance and resistance training. Abstract : Functional overload is known as a model for inducing muscle hypertrophy in rodents. It has been reported that endurance exercise (EE) suppresses muscle hypertrophy, and we investigated the effect of EE time on hypertrophied muscles. Long‐duration EE during functional overload does not suppress muscle hypertrophy but causes increased protein degradation and oxidative stress.