Novel guanidine compounds inhibit platelet‐derived growth factor receptor alpha transcription and oligodendrocyte precursor cell proliferation. Issue 3 (24th October 2020)

• Record Type: Journal Article
• Authors: Medved, Jelena; Wood, William M.; van Heyst, Michael D.; Sherafat, Amin; Song, Ju‐Young; Sakya, Sagune; Wright, Dennis L.; Nishiyama, Akiko
• Is Part Of: Glia. Volume 69:Issue 3(2021)
• Page Start: 792
• Page End: 811
• Publication Date: 2020-10-24
• Subjects: glioma -- NG2 -- oligodendrocyte precursor -- PDGF receptor -- platelet‐derived growth factor -- proliferation; Neuroglia -- Periodicals; Neurology -- Periodicals; 611.0188
• DOI: 10.1002/glia.23930
• Languages: English
• ISSNs: 0894-1491

Abstract

Abstract: Oligodendrocyte precursor cells (OPCs), also known as NG2 cells or polydendrocytes, are distributed widely throughout the developing and mature central nervous system. They remain proliferative throughout life and are an important source of myelinating cells in normal and demyelinating brain as well as a source of glioma, the most common type of primary brain tumor with a poor prognosis. OPC proliferation is dependent on signaling mediated by platelet‐derived growth factor (PDGF) AA binding to its alpha receptor (PDGFRα). Here, we describe a group of structurally related compounds characterized by the presence of a basic guanidine group appended to an aromatic core that is effective in specifically repressing the transcription of Pdgfra but not the related beta receptor (Pdgfrb) in OPCs. These compounds specifically and dramatically reduced proliferation of OPCs but not that of astrocytes and did not affect signal transduction by PDGFRα. These findings suggest that the compounds could be further developed for potential use in combinatorial treatment strategies for neoplasms with dysregulated PDGFRα function. Main Points: Structurally related guanidine compounds repress Pdgfra transcription. The guanidine compounds severely inhibit proliferation of OPCs but not astrocytes. The guanidine compounds do not affect PDGFRα‐mediated intracellular signaling.