Highly Sensitive Impedimetric Biosensor Based on Thermolysin Immobilized on a GCE Modified with AuNP‐decorated Graphene for the Detection of Ochratoxin A. Issue 1 (2nd September 2020)

• Record Type: Journal Article
• Authors: Zinoubi, Khaoula; Chrouda, Amani; Soltane, Raya; Al‐Ghamdi, Youssef O.; Garallah Almalki, Sami; Osman, Gamal; Barhoumi, Houcine; Jaffrezic Renault, Nicole
• Is Part Of: Electroanalysis. Volume 33:Issue 1(2021)
• Page Start: 136
• Page End: 145
• Publication Date: 2020-09-02
• Subjects: Ochratoxin A -- Thermolysin -- Electrochemical impedance spectroscopy -- Biosensor; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.202060247
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: The fabrication of a thermolysin‐based biosensor capable of detecting ochratoxin A (OTA) from food samples is described. The electrochemical deposition of calcium cross‐linked cellulose film (CCLC) and gold nanoparticles (AuNPs) on graphene (GR) for modification of a glassy carbon electrode (GCE) is the first step. Then the thermolysin (TLN) enzyme in a polyvinyl alcohol (PVA)/polyethylenimine (PEI) matrix is immobilized. The impedimetric biosensor response is linear from 0.2 nM to 100 nM with a detection limit of 0.2 nM. The obtained stable and reproducible biosensor is then applied for the detection of OTA in spiked extracts from coffee beans. Abstract :