Pathogenicity and toxicity of Fusarium tucumaniae and Fusarium crassistipitatum to soybean and Arabidopsis thaliana. (4th October 2020)

• Record Type: Journal Article
• Authors: Rosati, Romina G.; Ramos, Rocío S.; Scandiani, María M.; Luque, Alicia G.; Spampinato, Claudia P.
• Is Part Of: Plant pathology. Volume 70:Number 2(2021)
• Page Start: 407
• Page End: 416
• Publication Date: 2020-10-04
• Subjects: cell‐free culture filtrates -- fungal disease -- Glycine max -- plant–pathogen interaction -- sudden death syndrome; Agricultural pests -- Periodicals; Plant diseases -- Periodicals; 632
• DOI: 10.1111/ppa.13286
• Languages: English
• ISSNs: 0032-0862

Abstract

Abstract: Sudden death syndrome (SDS) of soybean is a fungal disease caused by at least four distinct Fusarium species: F . tucumaniae, F . virguliforme, F . brasiliense, and F . crassistipitatum . All four species are present in Argentina. These fungi are soilborne pathogens that only colonize roots and cause root necrosis. However, damage also reaches the aboveground part of the plant, and foliar chlorosis and necrosis, followed by premature defoliation, can be observed. Although the pathogenicity and phytotoxicity of F . virguliforme has been well characterized, knowledge regarding disease development by other fungal species is scarce. In this study, two plant species, soybean ( Glycine max ) and Arabidopsis thaliana, and isolates from two fungal species, F . tucumaniae and F . crassistipitatum, were used to comparatively analyse the fungal pathogenicity and the phytotoxicity of volatile organic compounds (VOCs) and cell‐free culture filtrates. Fungal inoculation had a significant effect on plant growth, regardless of the plant species. In addition, infected soybean plants showed disease incidence and foliar and root symptoms. Inhibition of A . thaliana growth was not due to VOCs emitted by fungi. Instead, both pathogens were shown to produce toxins that caused typical SDS foliar symptoms in soybean and root length reduction in A . thaliana . As far as we know, this is the first report that demonstrates that F . tucumaniae and F . crassistipitatum affect A . thaliana growth and emit VOCs, and that F . crassistipitatum produces toxins. Abstract : Both Fusarium tucumaniae and F. crassistipitatum fungal pathogens and their culture filtrates induced sudden death syndrome symptoms in soybean and negatively affected root growth in Arabidopsis thaliana .